Phosphate Buffered Saline - 10ml

Phosphate Buffered Saline – 10ml

$9.95

For research purposes only. Not for human or animal use & not FDA-approved. By purchasing, you confirm you are 21 or older and qualified researcher.

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Description

Phosphate Buffered Saline (Non-Biologix)

Research-Grade Buffer Solution (PBS)
Tagline: Standard Laboratory Buffer for Biological Research

Product Description

Phosphate Buffered Saline (PBS) is a balanced salt buffer solution widely used in biological and biochemical research laboratories. PBS is an aqueous isotonic solution of phosphate and saline, designed to maintain a stable pH and osmotic balance similar to physiological conditions. It contains salts such as sodium chloride, sodium phosphate, and potassium phosphate, providing a gentle environment compatible with many experimental systems.

This buffer solution is commonly used in cell culture workflows, molecular biology protocols, and general laboratory procedures where maintaining a neutral pH and isotonic environment is critical.

For Laboratory and Scientific Research Use Only. Not for Human Consumption.

Key Features

  • Stable physiological pH (~7.2–7.4)

  • Isotonic salt composition similar to biological fluids

  • Non-toxic to cells in short-term applications

  • Water-soluble and easy to use

  • Suitable for a range of biological assays

  • Available as ready-to-use liquid or concentrate

Important Note

For laboratory and scientific research only. Not for human consumption, diagnostic, or therapeutic use.

Details

Product Name Phosphate Buffered Saline (PBS)
Brand Non-Biologix
Buffer Type Phosphate Buffered Saline
pH ~7.2–7.4
Solubility Water-soluble
Storage Room temp or 2–8 °C

Research

Research Applications

Cell Culture Support

PBS is routinely used to wash cultured cells, remove residual media, and prepare cells for staining or fixation steps.

Buffering & Dilution

Maintains stable pH during dilution of biological reagents and sample preparation for downstream assays.

Molecular Biology Workflows

Used in DNA/RNA extraction, protein handling, immunoassays, and other methods where a physiologically balanced buffer is required.

References

  1. Phosphate-buffered saline (PBS): Overview of composition and uses in laboratory research.
    https://en.wikipedia.org/wiki/Phosphate-buffered_saline

  2. What is PBS solution? Uses, composition, and laboratory relevance.
    https://biologyinsights.com/what-is-pbs-solution-its-uses-and-composition/

  3. Phosphate Buffered Saline in biological workflows (Merck Millipore overview).
    https://www.merckmillipore.com/EC/en/products/chemistry-and-biochemicals/biochemicals/biological-buffers/phosphate-buffer-saline-pbs

  4. Phosphate buffered saline buffer details — composition and properties.
    https://www.interchim.fr/ft/6/68723A.pdf

Mechanism of Action

Mechanism of Action
Phosphate Buffer–Based pH Regulation

Phosphate Buffered Saline (PBS) functions through a phosphate buffer system composed of monosodium and disodium phosphate salts. This buffering system resists fluctuations in hydrogen ion concentration, allowing PBS to maintain a stable pH range of approximately 7.2–7.4 during laboratory procedures.

Ionic Strength and Osmotic Balance

The sodium chloride and potassium chloride components of PBS establish an ionic strength and osmolarity similar to physiological conditions. This isotonic environment helps minimize osmotic stress on cells and biological samples during short-term exposure in experimental workflows.

Chemical Inertness in Biological Systems

PBS does not actively participate in biochemical reactions. Instead, it provides a chemically inert aqueous environment that supports biological materials without altering protein structure, nucleic acids, or cellular components under standard laboratory conditions.

Compatibility With Biological Materials

Due to its balanced salt composition and neutral pH, PBS is broadly compatible with cells, tissues, proteins, antibodies, and nucleic acids. This compatibility allows PBS to function as a supportive medium for washing, dilution, and handling steps in biological research.

References

  1. Phosphate-buffered saline (PBS): Overview of composition and uses in laboratory research.
    https://en.wikipedia.org/wiki/Phosphate-buffered_saline

  2. What is PBS solution? Uses, composition, and laboratory relevance.
    https://biologyinsights.com/what-is-pbs-solution-its-uses-and-composition/

  3. Phosphate Buffered Saline in biological workflows (Merck Millipore overview).
    https://www.merckmillipore.com/EC/en/products/chemistry-and-biochemicals/biochemicals/biological-buffers/phosphate-buffer-saline-pbs

  4. Phosphate buffered saline buffer details — composition and properties.
    https://www.interchim.fr/ft/6/68723A.pdf

Certificate of Authenticity

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