Phosphate Buffered Saline (Biologix) 100ml

$14.95

For research purposes only. Not for human or animal use & not FDA-approved. By purchasing, you confirm you are 21 or older and qualified researcher.

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Description

Phosphate Buffered Saline (PBS) – Biologix (100 mL)

Research-Grade Isotonic Buffer Solution
Tagline: Cell Handling & Biological Stability Research


Product Description

Phosphate Buffered Saline (PBS) is a widely used isotonic, non-toxic buffer solution designed to maintain physiological pH and osmolarity in biological research applications. This Biologix PBS (100 mL) formulation is suitable for a broad range of cell culture, molecular biology, protein handling, and laboratory preparation procedures.

PBS is commonly used to wash cells and tissues, dilute reagents, reconstitute biomolecules, and maintain ionic balance without interfering with biological activity. Its buffering capacity makes it essential for experiments requiring stable pH conditions.

For Laboratory and Scientific Research Use Only. Not for Human Consumption.


Why Researchers Choose PBS (Biologix)
  • Physiological pH buffering for sensitive biological systems

  • Isotonic formulation minimizes osmotic stress on cells

  • Compatible with most biological assays and reagents

  • Ready-to-use sterile solution for convenience and consistency

  • Manufactured by Biologix, a trusted laboratory consumables supplier


Important Note

For laboratory and scientific research only. Not for human consumption, diagnostic, or therapeutic use.

Details

Product Name Phosphate Buffered Saline (PBS)
Solution Type Isotonic phosphate buffer
pH Range 7.2 – 7.4
Osmolarity ~300 mOsm/L
Form Sterile aqueous solution
Intended Research Use Cell washing, reagent dilution, buffer preparation

Research

Research Applications
Cell Culture & Tissue Handling

PBS is used extensively to wash cells and tissues without disrupting membrane integrity or cellular function. Its isotonic nature makes it ideal for maintaining cell viability during handling and transfer procedures.

Protein & Peptide Preparation

PBS provides a stable environment for diluting proteins, peptides, and antibodies, helping preserve structural integrity and biological activity during experiments.

Molecular Biology & Assay Preparation

PBS is compatible with many immunoassays, staining protocols, and enzymatic reactions, serving as a neutral buffer that does not interfere with biochemical processes.

General Laboratory Buffering

Researchers use PBS to maintain consistent ionic strength and pH, ensuring reproducibility across experimental conditions.


References

  1. Freshney RI. (2015). Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley-Blackwell.
    https://onlinelibrary.wiley.com/doi/book/10.1002/9781118873656

  2. Sambrook J, Russell DW. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
    https://cshlpress.com/default.tpl?action=full&–eqskudatarq=353

  3. Thermo Fisher Scientific. Phosphate-Buffered Saline (PBS) Overview and Applications.
    https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/basic-techniques/phosphate-buffered-saline.html

Mechanism of Action

Mechanism of Action
  • Phosphate buffering system: Maintains stable pH near physiological levels

  • Isotonic salt composition: Prevents osmotic stress on cells

  • Chemical inertness: Does not react with proteins, enzymes, or nucleic acids

  • Ionic balance support: Preserves biological structure and function during handling

  • Environmental stability: Provides consistent experimental conditions


References
  1. Freshney RI. (2015). Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley-Blackwell.
    https://onlinelibrary.wiley.com/doi/book/10.1002/9781118873656

  2. Sambrook J, Russell DW. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
    https://cshlpress.com/default.tpl?action=full&–eqskudatarq=353

  3. Thermo Fisher Scientific. Phosphate-Buffered Saline (PBS) Overview and Applications.
    https://www.thermofisher.com/us/en/home/references/protocols/cell-culture/basic-techniques/phosphate-buffered-saline.html